E1105 Preservative

E1105 crystals have been used to grow other functional materials for catalysis and biomedical applications. E1105 is a commonly used enzyme for lysing gram-positive bacteria. Due to the unique function of E1105 in which it can digest the cell wall and causes osmotic shock (burst the cell by suddenly changing solute concentration around the cell and thus the osmotic pressure), E1105 is commonly used in a lab setting to release proteins from bacterium periplasm while the inner membrane remains sealed as vesicles called the spheroplast.

For example, E. coli can be lysed using E1105 to free the contents of the periplasmic space. It is especially useful in a lab setting for trying to collect the contents of the periplasm.E1105 treatment is optimal at particular temperatures, pH ranges, and salt concentrations. E1105 activity increases with increasing temperatures, up to 60 degrees Celsius, with a pH range of 6.0-7.0. The salts present also affect E1105 treatment, where some assert inhibitory effects, and others promote lysis via E1105 treatment. Sodium chloride induces lysis, but at high concentrations, it is an active inhibitor of lysis. Similar observations have been seen with the use of potassium salts. Slight variations are present due to differences in bacterial strains.